A8181
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A8181
Sigma
Amido Black Staining Solution 2X
electrophoresis reagent
| Synonym: | Amido Black 10B, Naphthol Blue Black solution |
| CAS Number: | 1064-48-8 |
| Empirical Formula (Hill Notation): | C22H14N6Na2O9S2 |
| Molecular Weight: | 616.49 |
| Beilstein Registry Number: | 5374263 |
| MDL number: | MFCD00004017 |
| PubChem Substance ID: | 24891210 |
Description
| Application | Naphthol Blue Black stain is designed for rapid staining of protein bands on nitrocellulose membranes (Western blots).1 Blots are stained for 1 min and then destained for 30 minutes in 25% (v/v) isopropanol, 10% (v/v) acetic acid. Used to detect proteins in the lower microgram range with a clear background. Naphthol Blue Black can also be used for staining PAGE gels.2 |
| Reconstitution | After dilution with deionized water to a final volume of 500 ml, solution will contain 0.1% (w/v) amido black, 25% (v/v) isopropanol and 10% (v/v) acetic acid. |
Safety
| Hazard Codes | Xi |
| Risk Statements | 10-36/37/38 |
| Safety Statements | 26-36 |
| RIDADR | UN 2924 3/PG 3 |
| WGK Germany | 2 |
| Flash Point(F) | 86 °F |
| Flash Point(C) | 30 °C |
References
| Cited Reference | 1. Gershoni, J.M. and Palade, G., Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to a positively charged membrane. Anal. Biochem. 124, 396-405, (1982) Abstract |
| 2. Wilson, C.M., Staining of proteins on gels: Comparisons of dyes and procedures. Meth. Enzymol. 91, 236-247, (1983) Abstract |
